abstract：:Previously, we identified long repeat sequences that are frequently associated with genome rearrangements, including copy number variation (CNV), in many diverse isolates of the human fungal pathogen Candida albicans (Todd et al., 2019). Here, we describe the rapid acquisition of novel, high copy number CNVs during ad...

journal_title：eLife

authors： Todd RT,Selmecki A

更新日期：2020-07-20 00:00:00

abstract：:Pattern completion, or the ability to retrieve stable neural activity patterns from noisy or partial cues, is a fundamental feature of memory. Theoretical studies indicate that recurrently connected auto-associative or discrete attractor networks can perform this process. Although pattern completion and attractor dyna...

journal_title：eLife

authors： Bolding KA,Nagappan S,Han BX,Wang F,Franks KM

更新日期：2020-07-14 00:00:00

abstract：:Peroxiredoxins are H2O2 scavenging enzymes that also carry out H2O2 signaling and chaperone functions. In yeast, the major cytosolic peroxiredoxin, Tsa1 is required for both promoting resistance to H2O2 and extending lifespan upon caloric restriction. We show here that Tsa1 effects both these functions not by scavengi...

journal_title：eLife

authors： Roger F,Picazo C,Reiter W,Libiad M,Asami C,Hanzén S,Gao C,Lagniel G,Welkenhuysen N,Labarre J,Nyström T,Grøtli M,Hartl M,Toledano MB,Molin M

更新日期：2020-07-14 00:00:00

abstract：:Genome-wide association studies identified the BIN1 locus as a leading modulator of genetic risk in Alzheimer's disease (AD). One limitation in understanding BIN1's contribution to AD is its unknown function in the brain. AD-associated BIN1 variants are generally noncoding and likely change expression. Here, we determ...

journal_title：eLife

authors： Voskobiynyk Y,Roth JR,Cochran JN,Rush T,Carullo NV,Mesina JS,Waqas M,Vollmer RM,Day JJ,McMahon LL,Roberson ED

更新日期：2020-07-13 00:00:00

abstract：:Tumors are composed of many different cell types including cancer cells, fibroblasts, and immune cells. Dissecting functional metabolic differences between cell types within a mixed population can be challenging due to the rapid turnover of metabolites relative to the time needed to isolate cells. To overcome this cha...

journal_title：eLife

authors： Lau AN,Li Z,Danai LV,Westermark AM,Darnell AM,Ferreira R,Gocheva V,Sivanand S,Lien EC,Sapp KM,Mayers JR,Biffi G,Chin CR,Davidson SM,Tuveson DA,Jacks T,Matheson NJ,Yilmaz O,Vander Heiden MG

更新日期：2020-07-10 00:00:00

abstract：:The gonadotropin-releasing hormone (GnRH) neurons exhibit pulse and surge modes of activity to control fertility. They also exhibit an unusual bipolar morphology comprised of a classical soma-proximal dendritic zone and an elongated secretory process that can operate as both a dendrite and an axon, termed a 'dendron'....

journal_title：eLife

authors： Wang L,Guo W,Shen X,Yeo S,Long H,Wang Z,Lyu Q,Herbison AE,Kuang Y

更新日期：2020-07-09 00:00:00

abstract：:V-ATPase is an energy converting enzyme, coupling ATP hydrolysis/synthesis in the hydrophilic V1 domain, with proton flow through the Vo membrane domain, via rotation of the central rotor complex relative to the surrounding stator apparatus. Upon dissociation from the V1 domain, the Vo domain of the eukaryotic V-ATPas...

journal_title：eLife

authors： Kishikawa JI,Nakanishi A,Furuta A,Kato T,Namba K,Tamakoshi M,Mitsuoka K,Yokoyama K

更新日期：2020-07-08 00:00:00

abstract：:Understanding temporal dynamics of COVID-19 symptoms could provide fine-grained resolution to guide clinical decision-making. Here, we use deep neural networks over an institution-wide platform for the augmented curation of clinical notes from 77,167 patients subjected to COVID-19 PCR testing. By contrasting Electroni...

journal_title：eLife

authors： Wagner T,Shweta F,Murugadoss K,Awasthi S,Venkatakrishnan AJ,Bade S,Puranik A,Kang M,Pickering BW,O'Horo JC,Bauer PR,Razonable RR,Vergidis P,Temesgen Z,Rizza S,Mahmood M,Wilson WR,Challener D,Anand P,Liebers M,Doct

更新日期：2020-07-07 00:00:00

abstract：:Temporal patterning of neural progenitors leads to the sequential production of diverse neurons. To understand how extrinsic cues influence intrinsic temporal programs, we studied Drosophila mushroom body progenitors (neuroblasts) that sequentially produce only three neuronal types: γ, then α'β', followed by αβ. Oppos...

journal_title：eLife

authors： Rossi AM,Desplan C

更新日期：2020-07-06 00:00:00

abstract：:Current models successfully describe the auditory cortical response to natural sounds with a set of spectro-temporal features. However, these models have hardly been linked to the ill-understood neurobiological changes that occur in the aging auditory cortex. Modelling the hemodynamic response to a rich natural sound ...

journal_title：eLife

authors： Erb J,Schmitt LM,Obleser J

更新日期：2020-07-03 00:00:00

abstract：:Surveillance strategies based on whole genome sequencing could help with the early identification and detection of new forms of drug-resistant gonorrhoea. ...

journal_title：eLife

authors： Medland N

更新日期：2020-06-30 00:00:00

abstract：: ...

journal_title：eLife

authors： MacKay CE,Leo MD,Fernández-Peña C,Hasan R,Yin W,Mata-Daboin A,Bulley S,Gammons J,Mancarella S,Jaggar JH

更新日期：2020-06-30 00:00:00

abstract：:In endoplasmic reticulum-associated protein degradation (ERAD), membrane proteins are ubiquitinated, extracted from the membrane, and degraded by the proteasome. The cytosolic ATPase Cdc48 drives extraction by pulling on polyubiquitinated substrates. How hydrophobic transmembrane (TM) segments are moved from the phosp...

journal_title：eLife

authors： Schmidt CC,Vasic V,Stein A

更新日期：2020-06-26 00:00:00

abstract：:Our understanding of nonlinear stimulus transformations by neural circuits is hindered by the lack of comprehensive yet interpretable computational modeling frameworks. Here, we propose a data-driven approach based on deep neural networks to directly model arbitrarily nonlinear stimulus-response mappings. Reformulatin...

journal_title：eLife

authors： Keshishian M,Akbari H,Khalighinejad B,Herrero JL,Mehta AD,Mesgarani N

更新日期：2020-06-26 00:00:00

abstract：:We have previously demonstrated that checkpoint kinase 2 (CHK2) is a critical negative regulator of androgen receptor (AR) transcriptional activity, prostate cancer (PCa) cell growth, and androgen sensitivity. We have now uncovered that the AR directly interacts with CHK2 and ionizing radiation (IR) increases this int...

journal_title：eLife

authors： Ta HQ,Dworak N,Ivey ML,Roller DG,Gioeli D

更新日期：2020-06-24 00:00:00

abstract：:We collated contact tracing data from COVID-19 clusters in Singapore and Tianjin, China and estimated the extent of pre-symptomatic transmission by estimating incubation periods and serial intervals. The mean incubation periods accounting for intermediate cases were 4.91 days (95%CI 4.35, 5.69) and 7.54 (95%CI 6.76, 8...

journal_title：eLife

authors： Tindale LC,Stockdale JE,Coombe M,Garlock ES,Lau WYV,Saraswat M,Zhang L,Chen D,Wallinga J,Colijn C

更新日期：2020-06-22 00:00:00

abstract：:Animals and humans replay neural patterns encoding trajectories through their environment, both whilst they solve decision-making tasks and during rest. Both on-task and off-task replay are believed to contribute to flexible decision making, though how their relative contributions differ remains unclear. We investigat...

journal_title：eLife

authors： Eldar E,Lièvre G,Dayan P,Dolan RJ

更新日期：2020-06-17 00:00:00

abstract：:Disturbance from whale-watching can cause significant behavioural changes with fitness consequences for targeted whale populations. However, the sensory stimuli triggering these responses are unknown, preventing effective mitigation. Here, we test the hypothesis that vessel noise level is a driver of disturbance, usin...

journal_title：eLife

authors： Sprogis KR,Videsen S,Madsen PT

更新日期：2020-06-16 00:00:00

abstract：:Microbial fitness screens are a key technique in functional genomics. We present an all-in-one solution, pyphe, for automating and improving data analysis pipelines associated with large-scale fitness screens, including image acquisition and quantification, data normalisation, and statistical analysis. Pyphe is versat...

journal_title：eLife

authors： Kamrad S,Rodríguez-López M,Cotobal C,Correia-Melo C,Ralser M,Bähler J

更新日期：2020-06-16 00:00:00

abstract：:The COVID-19 pandemic has resulted in school closures and distancing requirements that have disrupted both work and family life for many. Concerns exist that these disruptions caused by the pandemic may not have influenced men and women researchers equally. Many medical journals have published papers on the pandemic, ...

journal_title：eLife

authors： Andersen JP,Nielsen MW,Simone NL,Lewiss RE,Jagsi R

更新日期：2020-06-15 00:00:00

abstract：:Many postdoctoral researchers apply for faculty positions knowing relatively little about the hiring process or what is needed to secure a job offer. To address this lack of knowledge about the hiring process we conducted a survey of applicants for faculty positions: the survey ran between May 2018 and May 2019, and r...

journal_title：eLife

authors： Fernandes JD,Sarabipour S,Smith CT,Niemi NM,Jadavji NM,Kozik AJ,Holehouse AS,Pejaver V,Symmons O,Bisson Filho AW,Haage A

更新日期：2020-06-12 00:00:00

abstract：:Sleep oscillations in the neocortex and hippocampus are critical for the integration of new memories into stable generalized representations in neocortex. However, the role of the thalamus in this process is poorly understood. To determine the thalamic contribution to non-REM oscillations (sharp-wave ripples, SWRs; sl...

journal_title：eLife

authors： Varela C,Wilson MA

更新日期：2020-06-11 00:00:00

abstract：:Struggling to get her research project up and running in a new country, a mother gets inspiration from her young daughter. ...

journal_title：eLife

authors： Sadanandappa MK

更新日期：2020-06-10 00:00:00

abstract：:Mu-opioid receptors (MORs) are crucial for analgesia by both exogenous and endogenous opioids. However, the distinct mechanisms underlying these two types of opioid analgesia remain largely unknown. Here, we demonstrate that analgesic effects of exogenous and endogenous opioids on inflammatory pain are mediated by MOR...

journal_title：eLife

authors： Zhang XY,Dou YN,Yuan L,Li Q,Zhu YJ,Wang M,Sun YG

更新日期：2020-06-10 00:00:00

abstract：:The genome-scale transcriptional programs that specify the mammalian trachea and esophagus are unknown. Though NKX2-1 and SOX2 are hypothesized to be co-repressive master regulators of tracheoesophageal fates, this is untested at a whole transcriptomic scale and their downstream networks remain unidentified. By combin...

journal_title：eLife

authors： Kuwahara A,Lewis AE,Coombes C,Leung FS,Percharde M,Bush JO

更新日期：2020-06-09 00:00:00

abstract：:Myelin destruction is followed by resident glia activation and mobilization of endogenous progenitors (OPC) which participate in myelin repair. Here we show that in response to demyelination, mature oligodendrocytes (OLG) bordering the lesion express Ndst1, a key enzyme for heparan sulfates (HS) synthesis. Ndst1+ OLG ...

journal_title：eLife

authors： Macchi M,Magalon K,Zimmer C,Peeva E,El Waly B,Brousse B,Jaekel S,Grobe K,Kiefer F,Williams A,Cayre M,Durbec P

更新日期：2020-06-09 00:00:00

abstract：:Proteins can self-organize into spatial patterns via non-linear dynamic interactions on cellular membranes. Modelling and simulations have shown that small GTPases can generate patterns by coupling guanine nucleotide exchange factors (GEF) to effectors, generating a positive feedback of GTPase activation and membrane ...

journal_title：eLife

authors： Cezanne A,Lauer J,Solomatina A,Sbalzarini IF,Zerial M

更新日期：2020-06-08 00:00:00

abstract：:Trimethylamine-oxide (TMAO) is present in seafood which is considered to be beneficial for health. Deep-water animals accumulate TMAO to protect proteins, such as lactate dehydrogenase (LDH), against hydrostatic pressure stress (HPS). We hypothesized that TMAO exerts beneficial effects on the circulatory system and pr...

journal_title：eLife

authors： Gawrys-Kopczynska M,Konop M,Maksymiuk K,Kraszewska K,Derzsi L,Sozanski K,Holyst R,Pilz M,Samborowska E,Dobrowolski L,Jaworska K,Mogilnicka I,Ufnal M

更新日期：2020-06-08 00:00:00

abstract：:Telomerase extends telomere sequences at chromosomal ends to protect genomic DNA. During this process it must select the correct nucleotide from a pool of nucleotides with various sugars and base pairing properties, which is critically important for the proper capping of telomeric sequences by shelterin. Unfortunately...

journal_title：eLife

authors： Schaich MA,Sanford SL,Welfer GA,Johnson SA,Khoang TH,Opresko PL,Freudenthal BD

更新日期：2020-06-05 00:00:00

abstract：:The human insular cortex is a heterogeneous brain structure which plays an integrative role in guiding behavior. The cytoarchitectonic organization of the human insula has been investigated over the last century using postmortem brains but there has been little progress in noninvasive in vivo mapping of its microstruc...

journal_title：eLife

authors： Menon V,Gallardo G,Pinsk MA,Nguyen VD,Li JR,Cai W,Wassermann D

更新日期：2020-06-04 00:00:00

abstract：:To move the body, the brain must precisely coordinate patterns of activity among diverse populations of motor neurons. Here, we use in vivo calcium imaging, electrophysiology, and behavior to understand how genetically-identified motor neurons control flexion of the fruit fly tibia. We find that leg motor neurons exhi...

journal_title：eLife

authors： Azevedo AW,Dickinson ES,Gurung P,Venkatasubramanian L,Mann RS,Tuthill JC

更新日期：2020-06-03 00:00:00

abstract：:Mechanical force is a determinant of Notch signalling but the mechanism of force detection and its coupling to Notch are unclear. We propose a role for Piezo1 channels, which are mechanically-activated non-selective cation channels. In cultured microvascular endothelial cells, Piezo1 channel activation by either shear...

journal_title：eLife

authors： Caolo V,Debant M,Endesh N,Futers TS,Lichtenstein L,Bartoli F,Parsonage G,Jones EA,Beech DJ

更新日期：2020-06-02 00:00:00

abstract：:Host-virus arms races are inherently asymmetric; viruses evolve much more rapidly than host genomes. Thus, there is high interest in discovering mechanisms by which host genomes keep pace with rapidly evolving viruses. One family of restriction factors, the APOBEC3 (A3) cytidine deaminases, has undergone positive sele...

journal_title：eLife

authors： Yang L,Emerman M,Malik HS,McLaughlin RN Jnr

更新日期：2020-06-01 00:00:00

abstract：:Peptidoglycan (PG) is the main component of bacterial cell walls and the target for many antibiotics. PG biosynthesis is tightly coordinated with cell wall growth and turnover, and many of these control activities depend upon PASTA-domain containing eukaryotic-like serine/threonine protein kinases (PASTA-eSTK) that se...

journal_title：eLife

authors： Wamp S,Rutter ZJ,Rismondo J,Jennings CE,Möller L,Lewis RJ,Halbedel S

更新日期：2020-05-29 00:00:00

abstract：:Mitochondrial dysfunction is associated with activation of the integrated stress response (ISR) but the underlying triggers remain unclear. We systematically combined acute mitochondrial inhibitors with genetic tools for compartment-specific NADH oxidation to trace mechanisms linking different forms of mitochondrial d...

journal_title：eLife

authors： Mick E,Titov DV,Skinner OS,Sharma R,Jourdain AA,Mootha VK

更新日期：2020-05-28 00:00:00

abstract：:Bone strength is determined by its dense cortical shell, generated by unknown mechanisms. Here we use the Dmp1Cre:Socs3f/f mouse, with delayed cortical bone consolidation, to characterise cortical maturation and identify control signals. We show that cortical maturation requires a reduction in cortical porosity, and a...

journal_title：eLife

authors： Walker EC,Truong K,McGregor NE,Poulton IJ,Isojima T,Gooi JH,Martin TJ,Sims NA

更新日期：2020-05-27 00:00:00

abstract：:AMPARs control fast synaptic communication between neurons and their function relies on auxiliary subunits, which importantly modulate channel properties. Although it has been suggested that AMPARs can bind to TARPs with variable stoichiometry, little is known about the effect that this stoichiometry exerts on certain...

journal_title：eLife

authors： Miguez-Cabello F,Sánchez-Fernández N,Yefimenko N,Gasull X,Gratacòs-Batlle E,Soto D

更新日期：2020-05-26 00:00:00

abstract：:Junín virus (JUNV) is one of five New World mammarenaviruses (NWMs) that causes fatal hemorrhagic disease in humans and is the etiological agent of Argentine hemorrhagic fever (AHF). The pathogenesis underlying AHF is poorly understood; however, a prolonged, elevated interferon-α (IFN-α) response is associated with a ...

journal_title：eLife

authors： Hickerson BT,Sefing EJ,Bailey KW,Van Wettere AJ,Penichet ML,Gowen BB

更新日期：2020-05-26 00:00:00

abstract：:The pressure for every research article to tell a clear story often leads researchers in the life sciences to exclude experiments that 'did not work' when they write up their results. However, this practice can lead to reporting bias if the decisions about which experiments to exclude are taken after data have been co...

journal_title：eLife

authors： Neves K,Amaral OB

更新日期：2020-05-22 00:00:00

abstract：:Anxiety results in sub-optimal motor learning, but the precise mechanisms through which this effect occurs remain unknown. Using a motor sequence learning paradigm with separate phases for initial exploration and reward-based learning, we show that anxiety states in humans impair learning by attenuating the update of ...

journal_title：eLife

authors： Sporn S,Hein T,Herrojo Ruiz M

更新日期：2020-05-19 00:00:00